ABSTRACT
Objective To investigate whether pyrrolidine dithiocarbamate (PDTC) can attenuate the acute radiation-induced heart damage (RIHD) by inhibiting the activation of NF-κB and its downstream signaling pathways in rat models. Methods Twenty-one male adult Sprague-Dawley (SD) rats were randomly divided into the blank control, irradiation and PDTC plus irradiation groups (n=7 for each group). In the irradiation and PDTC+ irradiation groups,the rats received 6 MV X-ray at a single fraction of 20.0 Gy. In the PDTC+ irradiation group, intraperitonal injection of PDTC was administered at a dose of 120 mg/kg body weight,30 minutes prior to radiation, once daily for 1-14 days. On the 14thday,pathological changes of myocardial tissue were observed. Masson's trichrome staining was performed to calculate the collagen volume fraction (CVF) of myocardial cells. The expression levels of NF-κB family members including p50, p65,HIF-1α,connective tissue growth factor (CTGF) and collagen type 1(COL-1) proteins and mRNA were quantitatively measured by Western blot and quantitative real-time PCR (qPCR). Statistical analysis was conducted by using t-test. Results HE staining demonstrated that compared with the irradiation group, the severity of myocardial edema was alleviated,the infiltration of inflammatory cells was mitigated and the quantity of fibroblasts was reduced in the PDTC+irradiation group. Masson's trichrome staining revealed that PDCT intervention could decrease the deposition of collagen fiber in the interstitial tissues. Semi-quantitative analysis demonstrated that the CVF value in the PDTC+irradiation group was (9.99± 0.32)%, significantly lower compared with (22.05±0.21)% in the irradiation group (P<0.05). Western blot and qRT-PCR demonstrated that the expression levels of p50,p65,and HIF-1αproteins and mRNA in the PDTC+ irradiation group were significantly down-regulated compared with those in the irradiation group (all P<0.05). Compared with the irradiation group,the expression levels of CTGF protein and mRNA tended to decline (all P>0.05),and the expression levels of COL-1 protein and mRNA were equally inclined to decrease (P<0.05 and P>0.05). Conclusion PDTC can alleviate the acute RIHD by suppressing the activation of NF-κB and its downstream HIF-1α transcription.
ABSTRACT
Objective To compare the effects of Chemiluminescence immunoassay ( CLIA) and ELISA for detecting the HIV antibody in order to explore the feasibility of CLIA.Methods 3,542 AIDS high-risk people were randomly selected from February 2013 to March 2015 in our city, the serum was collected and HIV antibodies in the serum were detected by CLIA and ELISA.The detection results were confirmed by the Western blot.Results By ELISA assay, 56 serum samples were positive with a positive rate of 1.58%, while CLIA 61 were positive with a pos-itive rate of 1.72%.The detection results on 11 serum samples were inconsistent among the 3,542 serum samples by ELISA and CLIA.The positive predictive value of CLIA was 96.72%, the detection sensitivity 98.33%and specific-ity 99.94%, while the positive predictive value of ELISA was 96.43%, the detection sensitivity 90.00%and speci-ficity 99.94%.The positive predictive value, sensitivity, specificity and other indicators showed no significant differ-ence between ELISA and CLIA(P>0.05).Conclusion CLIA shows high sensitivity, specificity and accuracy and therefore it can be used for detecting HIV antibody.